SPED Light Sheet Microscopy: Fast Mapping of Biological System Structure and Function.

ABSTRACT: The goal of understanding living nervous systems has driven interest in high-speed and large field-of-view volumetric imaging at cellular resolution. Light sheet microscopy approaches have emerged for cellular-resolution functional brain imaging in small organisms such as larval zebrafish, but remain fundamentally limited in speed. Here, we have developed SPED light sheet microscopy, which combines large volumetric field-of-view via an extended depth of field with the optical sectioning of light sheet microscopy, thereby eliminating the need to physically scan detection objectives for volumetric imaging. SPED enables scanning of thousands of volumes-per-second, limited only by camera acquisition rate, through the harnessing of optical mechanisms that normally result in unwanted spherical a

SUBMITTER: Tomer R 

PROVIDER: S-EPMC4775738 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature