Multiplexed Intact-Tissue Transcriptional Analysis at Cellular Resolution.

ABSTRACT: In recently developed approaches for high-resolution imaging within intact tissue, molecular characterization over large volumes has been largely restricted to labeling of proteins. But volumetric nucleic acid labeling may represent a far greater scientific and clinical opportunity, enabling detection of not only diverse coding RNA variants but also non-coding RNAs. Moreover, scaling immunohistochemical detection to large tissue volumes has limitations due to high cost, limited renewability/availability, and restricted multiplexing capability of antibody labels. With the goal of versatile, high-content, and scalable molecular phenotyping of intact tissues, we developed a method using carbodiimide-based chemistry to stably retain RNAs in clarified tissue, coupled with amplification tools fo

SUBMITTER: Sylwestrak EL 

PROVIDER: S-EPMC4775740 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature