Resolution and Probabilistic Models of Components in CryoEM Maps of Mature P22 Bacteriophage.

ABSTRACT: CryoEM continues to produce density maps of larger and more complex assemblies with multiple protein components of mixed symmetries. Resolution is not always uniform throughout a cryoEM map, and it can be useful to estimate the resolution in specific molecular components of a large assembly. In this study, we present procedures to 1) estimate the resolution in subcomponents by gold-standard Fourier shell correlation (FSC); 2) validate modeling procedures, particularly at medium resolutions, which can include loop modeling and flexible fitting; and 3) build probabilistic models that combine high-accuracy priors (such as crystallographic structures) with medium-resolution cryoEM densities. As an example, we apply these methods to new cryoEM maps of the mature bacteriophage P22, reconstructed

SUBMITTER: Pintilie G 

PROVIDER: S-EPMC4775875 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature