Topographic Cues Reveal Two Distinct Spreading Mechanisms in Blood Platelets.

ABSTRACT: Blood platelets are instrumental in blood clotting and are thus heavily involved in early wound closure. After adhering to a substrate they spread by forming protrusions like lamellipodia and filopodia. However, the interaction of these protrusions with the physical environment of platelets while spreading is not fully understood. Here we dynamically image platelets during this spreading process and compare their behavior on smooth and on structured substrates. In particular we analyze the temporal evolution of the spread area, the cell morphology and the dynamics of individual filopodia. Interestingly, the topographic cues enable us to distinguish two spreading mechanisms, one that is based on numerous persistent filopodia and one that rather involves lamellipodia. Filopodia-driven spread

SUBMITTER: Sandmann R 

PROVIDER: S-EPMC4776100 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature