Differential root transcriptomics in a polyploid non-model crop: the importance of respiration during osmotic stress.

ABSTRACT: To explore the transcriptomic global response to osmotic stress in roots, 18 mRNA-seq libraries were generated from three triploid banana genotypes grown under mild osmotic stress (5% PEG) and control conditions. Illumina sequencing produced 568 million high quality reads, of which 70-84% were mapped to the banana diploid reference genome. Using different uni- and multivariate statistics, 92 genes were commonly identified as differentially expressed in the three genotypes. Using our in house workflow to analyze GO enriched and underlying biochemical pathways, we present the general processes affected by mild osmotic stress in the root and focus subsequently on the most significantly overrepresented classes associated with: respiration, glycolysis and fermentation. We hypothesize that in fa

SUBMITTER: Zorrilla-Fontanesi Y 

PROVIDER: S-EPMC4776286 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature