Transcription-associated processes cause DNA double-strand breaks and translocations in neural stem/progenitor cells.

ABSTRACT: High-throughput, genome-wide translocation sequencing (HTGTS) studies of activated B cells have revealed that DNA double-strand breaks (DSBs) capable of translocating to defined bait DSBs are enriched around the transcription start sites (TSSs) of active genes. We used the HTGTS approach to investigate whether a similar phenomenon occurs in primary neural stem/progenitor cells (NSPCs). We report that breakpoint junctions indeed are enriched around TSSs that were determined to be active by global run-on sequencing analyses of NSPCs. Comparative analyses of transcription profiles in NSPCs and B cells revealed that the great majority of TSS-proximal junctions occurred in genes commonly expressed in both cell types, possibly because this common set has higher transcription levels on average th

SUBMITTER: Schwer B 

PROVIDER: S-EPMC4776469 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature