In vivo fluorescence lifetime imaging for monitoring the efficacy of the cancer treatment.

ABSTRACT:

Purpose

Advances in tumor biology created a foundation for targeted therapy aimed at inactivation of specific molecular mechanisms responsible for cell malignancy. In this paper, we used in vivo fluorescence lifetime imaging with HER2-targeted fluorescent probes as an alternative imaging method to investigate the efficacy of targeted therapy with 17-DMAG (an HSP90 inhibitor) on tumors with high expression of HER2 receptors.

Experimental design

HER2-specific Affibody, conjugated to Alexafluor 750, was injected into nude mice bearing HER2-positive tumor xenograft. The fluorescence lifetime was measured before treatment and monitored after the probe injections at 12 hours after the last treatment dose, when the response to the 17-DMAG therapy was the most pronounced as well as

SUBMITTER: Ardeshirpour Y 

PROVIDER: S-EPMC4776631 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature