Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-κB signaling pathway.

ABSTRACT:

Objective

To investigate the effects of papaverine (PAP) on lipopolysaccharide (LPS)-induced microglial activation and its possible mechanisms.

Materials and methods

BV2 microglial cells were first pretreated with PAP (0, 0.4, 2, 10, and 50 μg/mL) and then received LPS stimulation. Transcription and production of proinflammatory factors (IL1β, TNFα, iNOS, and COX-2) were used to evaluate microglial activation. The transcriptional changes undergone by M1/M2a/M2b markers were used to evaluate phenotype transformation of BV2 cells. Immunofluorescent staining and Western blot were used to detect the location and expression of P65 and p-IKK in the presence or absence of PAP pretreatment.

Results

Pretreatment with PAP significantly inhibited the expression of IL1β and TNFα,

SUBMITTER: Dang Y 

PROVIDER: S-EPMC4777259 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature