Characterization of the expression of the pro-metastatic Mena(INV) isoform during breast tumor progression.

ABSTRACT: Several functionally distinct isoforms of the actin regulatory Mena are produced by alternative splicing during tumor progression. Forced expression of the Mena(INV) isoform drives invasion, intravasation and metastasis. However, the abundance and distribution of endogenously expressed Mena(INV) within primary tumors during progression remain unknown, as most studies to date have only assessed relative mRNA levels from dissociated tumor samples. We have developed a Mena(INV) isoform-specific monoclonal antibody and used it to examine Mena(INV) expression patterns in mouse mammary and human breast tumors. Mena(INV) expression increases during tumor progression and to examine the relationship between Mena(INV) expression and markers for epithelial or mesenchymal status, stemness, stromal cel

SUBMITTER: Oudin MJ 

PROVIDER: S-EPMC4777680 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature