High-throughput discovery of post-transcriptional cis-regulatory elements.

ABSTRACT:

Background

Post-transcriptional gene regulation controls the amount of protein produced from an individual mRNA by altering rates of decay and translation. Many sequence elements that direct post-transcriptional regulation have been found; in mammals, most such elements are located within the 3' untranslated regions (3'UTRs). Comparative genomic studies demonstrate that mammalian 3'UTRs contain extensive conserved sequence tracts, yet only a small fraction corresponds to recognized elements, implying that many additional novel elements exist. Despite a variety of computational, molecular, and biochemical approaches, identifying functional 3'UTRs elements remains difficult.

Results

We created a high-throughput cell-based screen that enables identification of functional post-t

SUBMITTER: Wissink EM 

PROVIDER: S-EPMC4778349 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature