Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy.

ABSTRACT:

Background

DNA hybridization is at the basis of most current technologies for genotyping and sequencing, due to the unique properties of DNA base-pairing that guarantee a high grade of selectivity. Nonetheless the presence of single base mismatches or not perfectly matched sequences can affect the response of the devices and the major challenge is, nowadays, to distinguish a mismatch of a single base and, at the same time, unequivocally differentiate devices read-out of fully and partially matching sequences.

Results

We present here two platforms based on different sensing strategies, to detect mismatched and/or perfectly matched complementary DNA strands hybridization into ssDNA oligonucleotide monolayers. The first platform exploits atomic force microscopy-based nanolithog

SUBMITTER: Ngavouka MDN 

PROVIDER: S-EPMC4778512 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature