Individual Cardiac Mitochondria Undergo Rare Transient Permeability Transition Pore Openings.

ABSTRACT:

Rationale

Mitochondria produce ATP, especially critical for survival of highly aerobic cells, such as cardiac myocytes. Conversely, opening of mitochondrial high-conductance and long-lasting permeability transition pores (mPTP) causes respiratory uncoupling, mitochondrial injury, and cell death. However, low conductance and transient mPTP openings (tPTP) might limit mitochondrial Ca(2+) load and be cardioprotective, but direct evidence for tPTP in cells is limited.

Objective

To directly characterize tPTP occurrence during sarcoplasmic reticulum Ca(2+) release in adult cardiac myocytes.

Methods and results

Here, we measured tPTP directly as transient drops in mitochondrial [Ca(2+)] ([Ca(2+)]mito) and membrane potential (ΔΨm) in adult cardiac myocytes during cyclic sarc

SUBMITTER: Lu X 

PROVIDER: S-EPMC4779382 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature