Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis.

ABSTRACT: The substrate specificity of enzymes from natural products' metabolism is a topic of considerable interest, with potential biotechnological use implicit in the discovery of promiscuous enzymes. However, such studies are often limited by the availability of substrates and authentic standards for identification of the resulting products. Here, a modular metabolic engineering system is used in a combinatorial biosynthetic approach toward alleviating this restriction. In particular, for studies of the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), which is involved in production of the diterpenoid plant hormone gibberellin. Many, but not all, plants make a variety of related diterpenes, whose structural similarity to ent-kaurene makes them potential substrates for KO. Use of comb

SUBMITTER: Mafu S 

PROVIDER: S-EPMC4780596 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature