Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography.

ABSTRACT: Cryo-electron tomography (Cryo-ET) is a powerful three-dimensional (3-D) imaging technique for visualizing macromolecular complexes in their native context at a molecular level. The technique involves initially preserving the sample in its native state by rapidly freezing the specimen in vitreous ice, then collecting a series of micrographs from different angles at high magnification, and finally computationally reconstructing a 3-D density map. The frozen-hydrated specimen is extremely sensitive to the electron beam and so micrographs are collected at very low electron doses to limit the radiation damage. As a result, the raw cryo-tomogram has a very low signal to noise ratio characterized by an intrinsically noisy image. To better visualize subjects of interest, conventional imaging anal

SUBMITTER: Morado DR 

PROVIDER: S-EPMC4781705 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature