A Novel Pentapeptide Targeting Integrin β3-Subunit Inhibits Platelet Aggregation and Its Application in Rat for Thrombosis Prevention.

ABSTRACT:

Background

Antiplatelet therapy plays a pivotal role in the prevention and treatment of thrombotic diseases. We reported the screening of P1C as a novel integrin-binding peptide from the C-terminal of connective tissue growth factor. Primary study indicated that P1C has potential against platelet aggregation.

Objectives

We aimed to find the shortest active unit from the P1C fragments and explore its in vivo and in vitro activities.

Methods

A series of truncated P1C fragments was prepared and screened for antiplatelet activity. The most active fragment was evaluated using coagulation assays. Flow cytometry and confocal microscopy were used to determine the interaction between the peptide and the integrin. The in vivo potential was further explored using two types of ra

SUBMITTER: Qu Q 

PROVIDER: S-EPMC4782163 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature