Characterization of various cell lines from different ampullary cancer subtypes and cancer associated fibroblast-mediated responses.

ABSTRACT:

Background

Ampullary cancer is a relatively rare form of cancer and usually treated by pancreatoduodenectomy, followed by adjuvant therapy. The intestinal subtype is associated with markedly improved prognosis after resection. At present, only few cell lines are available for in vitro studies of ampullary cancer and they have not been collectively characterized.

Methods

We characterize five ampullary cancer cell lines by subtype maker expression, epithelial-mesenchymal transition (EMT) features, growth and invasion, drug sensitivity and response to cancer-associated fibroblast conditioned medium (CAF-CM).

Results

On the basis of EMT features, subtype marker expression, growth, invasion and drug sensitivity three types of cell lines could be distinguished: mesenchymal-

SUBMITTER: Lai ZW 

PROVIDER: S-EPMC4782372 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature