Large-scale time series microscopy of neovessel growth during angiogenesis.

ABSTRACT: During angiogenesis, growing neovessels must effectively navigate through the tissue space as they elongate and subsequently integrate into a microvascular network. While time series microscopy has provided insight into the cell activities within single growing neovessel sprouts, less is known concerning neovascular dynamics within a large angiogenic tissue bed. Here, we developed a time-lapse imaging technique that allowed visualization and quantification of sprouting neovessels as they form and grow away from adult parent microvessels in three dimensions over cubic millimeters of matrix volume during the course of up to 5 days on the microscope. Using a new image acquisition procedure and novel morphometric analysis tools, we quantified the elongation dynamics of growing neovessels and f

SUBMITTER: Utzinger U 

PROVIDER: S-EPMC4782613 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature