Product Rearrangement from Altering a Single Residue in the Rice syn-Copalyl Diphosphate Synthase.

ABSTRACT: Through site-directed mutagenesis targeted at identification of the catalytic base in the rice (Oryza sativa) syn-copalyl diphosphate synthase OsCPS4, changes to a single residue (H501) were found to induce rearrangement rather than immediate deprotonation of the initially formed bicycle, leading to production of the novel compound syn-halimadienyl diphosphate. These mutational results are combined with quantum chemical calculations to provide insight into the underlying reaction mechanism.

SUBMITTER: Potter KC 

PROVIDER: S-EPMC4782720 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature