Full-length nucleotide sequences of 30 common SLC44A2 alleles encoding human neutrophil antigen-3.

ABSTRACT:

Background

Human neutrophil antigen-3a (HNA-3a) alloantibodies can cause severe transfusion-related acute lung injury. The frequencies of the single-nucleotide polymorphisms (SNPs) indicative of the two clinically relevant HNA-3a/b antigens are known in many populations. In this study, we determined the full-length nucleotide sequence of common SLC44A2 alleles encoding the choline transporter-like protein-2 that harbors HNA-3a/b antigens.

Study design and methods

A method was devised to determine the full-length coding sequence (CDS) and adjacent intron sequences from genomic DNA by eight polymerase chain reaction amplifications covering all 22 SLC44A2 exons. Samples from 200 African American, 96 Caucasian, two Hispanic, and four Asian blood donors were analyzed. We develope

SUBMITTER: Chen Q 

PROVIDER: S-EPMC4783217 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature