CD19 controls Toll-like receptor 9 responses in human B cells.

ABSTRACT:

Background

CD19 is a B cell-specific molecule that serves as a major costimulatory molecule for amplifying B-cell receptor (BCR) responses. Biallelic CD19 gene mutations cause common variable immunodeficiency in human subjects. BCR- and Toll-like receptor (TLR) 9-induced B-cell responses are impaired in most patients with common variable immunodeficiency.

Objective

We sought to analyze whether CD19 is required for TLR9 function in human B cells.

Methods

Expression of surface activation markers was assessed after anti-IgM or CpG stimulation by using flow cytometry on B cells from patients with 1 or 2 defective CD19 alleles, which decrease or abrogate CD19 expression, respectively. The phosphorylation or interaction of signaling molecules was analyzed by using phospho f

SUBMITTER: Morbach H 

PROVIDER: S-EPMC4783287 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature