A novel metabolomic approach used for the comparison of Staphylococcus aureus planktonic cells and biofilm samples.

ABSTRACT:

Introduction

Bacterial cell characteristics change significantly during differentiation between planktonic and biofilm states. While established methods exist to detect and identify transcriptional and proteomic changes, metabolic fluctuations that distinguish these developmental stages have been less amenable to investigation.

Objectives

The objectives of the study were to develop a robust reproducible sample preparation methodology for high throughput biofilm analysis and to determine differences between Staphylococcus aureus in planktonic and biofilm states.

Methods

The method uses bead beating in a chloroform/methanol/water extraction solvent to both disrupt cells and quench metabolism. Verification of the method was performed using liquid-chromatography-mass spec

SUBMITTER: Stipetic LH 

PROVIDER: S-EPMC4783440 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature