In vivo reprogramming reactive glia into iPSCs to produce new neurons in the cortex following traumatic brain injury.

ABSTRACT: Traumatic brain injury (TBI) results in a significant amount of cell death in the brain. Unfortunately, the adult mammalian brain possesses little regenerative potential following injury and little can be done to reverse the initial brain damage caused by trauma. Reprogramming adult cells to generate induced pluripotent stem cell (iPSCs) has opened new therapeutic opportunities to generate neurons in a non-neurogenic regions in the cortex. In this study we showed that retroviral mediated expression of four transcription factors, Oct4, Sox2, Klf4, and c-Myc, cooperatively reprogrammed reactive glial cells into iPSCs in the adult neocortex following TBI. These iPSCs further differentiated into a large number of neural stem cells, which further differentiated into neurons and glia in situ, an

SUBMITTER: Gao X 

PROVIDER: S-EPMC4783661 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature