Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments.

ABSTRACT: The microenvironment determines cell behavior, but the underlying molecular mechanisms are poorly understood because quantitative studies of cell signaling and behavior have been challenging due to insufficient spatial and/or temporal resolution and limitations on microenvironmental control. Here we introduce microenvironmental selective plane illumination microscopy (meSPIM) for imaging and quantification of intracellular signaling and submicrometer cellular structures as well as large-scale cell morphological and environmental features. We demonstrate the utility of this approach by showing that the mechanical properties of the microenvironment regulate the transition of melanoma cells from actin-driven protrusion to blebbing, and we present tools to quantify how cells manipulate individ

SUBMITTER: Welf ES 

PROVIDER: S-EPMC4784259 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature