Ultra-precise detection of mutations by droplet-based amplification of circularized DNA.

ABSTRACT:

Background

NGS (next generation sequencing) has been widely used in studies of biological processes, ranging from microbial evolution to cancer genomics. However, the error rate of NGS (0.1 % ~ 1 %) is still remaining a great challenge for comprehensively investigating the low frequency variations, and the current solution methods have suffered severe amplification bias or low efficiency.

Results

We creatively developed Droplet-CirSeq for relatively efficient, low-bias and ultra-sensitive identification of variations by combining millions of picoliter uniform-sized droplets with Cir-seq. Droplet-CirSeq is entitled with an incredibly low error rate of 3 ~ 5 X 10(-6). To systematically evaluate the performances of amplification uniformity and capability of mutation identificat

SUBMITTER: Wang K 

PROVIDER: S-EPMC4784281 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature