De novo assembly and analysis of the transcriptome of Ocimum americanum var. pilosum under cold stress.

ABSTRACT:

Background

Ocimum americanum var. pilosum is a chilling-sensitive, widely distributed plant that is consumed as a vegetable in central and southern China. To increase our understanding of cold stress responses in this species, we performed de novo transcriptome assembly for O. americanum var. pilosum and compared the transcriptomes of plants grown under normal and low temperatures.

Results

A total of 115,022,842 high quality, clean reads were obtained from four libraries (two replicates of control samples and two replicates of chilling-treated samples) and were used to perform de novo transcriptome assembly. After isoforms were considered, 42,816 unigenes were generated, 30,748 of which were similar to known proteins as determined by a BLASTx search (E-value < =1.0E-05) agai

SUBMITTER: Zhan X 

PROVIDER: S-EPMC4784345 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature