New molecular settings to support in vivo anti-malarial assays.

ABSTRACT:

Background

Quantitative real-time PCR (qPCR) is now commonly used as a method to confirm diagnosis of malaria and to differentiate recrudescence from re-infection, especially in clinical trials and in reference laboratories where precise quantification is critical. Although anti-malarial drug discovery is based on in vivo murine efficacy models, use of molecular analysis has been limited. The aim of this study was to develop qPCR as a valid methodology to support pre-clinical anti-malarial models by using filter papers to maintain material for qPCR and to compare this with traditional methods.

Methods

FTA technology (Whatman) is a rapid and safe method for extracting nucleic acids from blood. Peripheral blood samples from mice infected with Plasmodium berghei, P. yoelii, or

SUBMITTER: Bahamontes-Rosa N 

PROVIDER: S-EPMC4784371 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature