LPS-induced production of TNF-α and IL-6 in mast cells is dependent on p38 but independent of TTP.

ABSTRACT: The production of the proinflammatory cytokines TNF-α and IL-6 is regulated by various mRNA-binding proteins, influencing stability and translation of the respective transcripts. Research in macrophages has shown the importance of the p38-MK2-tristetraprolin (TTP) axis for regulation of TNF-α mRNA stability and translation. In the current study we examined a possible involvement of p38 and TTP in LPS-induced cytokine production in bone marrow-derived mast cells (BMMCs). Using pharmacological inhibitors we initially found a strong dependence of LPS-induced TNF-α and IL-6 production on p38 activation, whereas activation of the Erk pathway appeared dispensable. LPS treatment also induced p38-dependent expression of the TTP gene. This prompted us to analyze the proinflammatory cytokine respons

SUBMITTER: Hochdorfer T 

PROVIDER: S-EPMC4784419 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature