Using selenomethionyl derivatives to assign sequence in low-resolution structures of the AP2 clathrin adaptor.

ABSTRACT: Selenomethionine incorporation is a powerful technique for assigning sequence to regions of electron density at low resolution. Genetic introduction of methionine point mutations and the subsequent preparation and crystallization of selenomethionyl derivatives permits unambiguous sequence assignment by enabling the placement of the anomalous scatterers (Se atoms) thus introduced. Here, the use of this approach in the assignment of sequence in a part of the AP2 clathrin adaptor complex that is responsible for clathrin binding is described. AP2 plays a pivotal role in clathrin-mediated endocytosis, a tightly regulated process in which cell-surface transmembrane proteins are internalized from the plasma membrane by incorporation into lipid-enclosed transport vesicles. AP2 binds cargo destined

SUBMITTER: Kelly BT 

PROVIDER: S-EPMC4784665 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature