Can I solve my structure by SAD phasing? Anomalous signal in SAD phasing.

ABSTRACT: A key challenge in the SAD phasing method is solving a structure when the anomalous signal-to-noise ratio is low. A simple theoretical framework for describing measurements of anomalous differences and the resulting useful anomalous correlation and anomalous signal in a SAD experiment is presented. Here, the useful anomalous correlation is defined as the correlation of anomalous differences with ideal anomalous differences from the anomalous substructure. The useful anomalous correlation reflects the accuracy of the data and the absence of minor sites. The useful anomalous correlation also reflects the information available for estimating crystallographic phases once the substructure has been determined. In contrast, the anomalous signal (the peak height in a model-phased anomalous differe

SUBMITTER: Terwilliger TC 

PROVIDER: S-EPMC4784666 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature