Using NMR spectroscopy to elucidate the role of molecular motions in enzyme function.

ABSTRACT: Conformational motions play an essential role in enzyme function, often facilitating the formation of enzyme-substrate complexes and/or product release. Although considerable debate remains regarding the role of molecular motions in the conversion of enzymatic substrates to products, numerous examples have found motions to be crucial for optimization of enzyme scaffolds, effective substrate binding, and product dissociation. Conformational fluctuations are often rate-limiting to enzyme catalysis, primarily through product release, with the chemical reaction occurring much more quickly. As a result, the direct involvement of motions at various stages along the enzyme reaction coordinate remains largely unknown and untested. In the following review, we describe the use of solution NMR techni

SUBMITTER: Lisi GP 

PROVIDER: S-EPMC4785347 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature