Insight into determinants of substrate binding and transport in a multidrug efflux protein.

ABSTRACT: Multidrug resistance arising from the activity of integral membrane transporter proteins presents a global public health threat. In bacteria such as Escherichia coli, transporter proteins belonging to the major facilitator superfamily make a considerable contribution to multidrug resistance by catalysing efflux of myriad structurally and chemically different antimicrobial compounds. Despite their clinical relevance, questions pertaining to mechanistic details of how these promiscuous proteins function remain outstanding, and the role(s) played by individual amino acid residues in recognition, binding and subsequent transport of different antimicrobial substrates by multidrug efflux members of the major facilitator superfamily requires illumination. Using in silico homology modelling, molec

SUBMITTER: Alegre KO 

PROVIDER: S-EPMC4785361 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature