In vivo visualization and quantification of collecting lymphatic vessel contractility using near-infrared imaging.

ABSTRACT: Techniques to image lymphatic vessel function in either animal models or in the clinic are limited. In particular, imaging methods that can provide robust outcome measures for collecting lymphatic vessel function are sorely needed. In this study, we aimed to develop a method to visualize and quantify collecting lymphatic vessel function in mice, and to establish an in vivo system for evaluation of contractile agonists and antagonists using near-infrared fluorescence imaging. The flank collecting lymphatic vessel in mice was exposed using a surgical technique and a near-infrared tracer was infused into the inguinal lymph node. Collecting lymphatic vessel contractility and valve function could be easily visualized after the infusion. A diameter tracking method was established and the diamete

SUBMITTER: Chong C 

PROVIDER: S-EPMC4785392 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature