A model of muscle atrophy based on live microscopy of muscle remodelling in Drosophila metamorphosis.

ABSTRACT: Genes controlling muscle size and survival play important roles in muscle wasting diseases. In Drosophila melanogaster metamorphosis, larval abdominal muscles undergo two developmental fates. While a doomed population is eliminated by cell death, another persistent group is remodelled and survives into adulthood. To identify and characterize genes involved in the development of remodelled muscles, we devised a workflow consisting of in vivo imaging, targeted gene perturbation and quantitative image analysis. We show that inhibition of TOR signalling and activation of autophagy promote developmental muscle atrophy in early, while TOR and yorkie activation are required for muscle growth in late pupation. We discovered changes in the localization of myonuclei during remodelling that involve a

SUBMITTER: Kuleesha Y 

PROVIDER: S-EPMC4785973 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature