Comparative Proteomic Identification of Mature and Immature Sperm in the Catfish Cranoglanis bouderius.

ABSTRACT: To understand the molecular responses of mature and immature sperm in the catfish Cranoglanis bouderius, we used the iTRAQ proteomics approach to perform proteomic profiling of spermatogenesis in C. bouderius. As a result, 1,941 proteins were identified, including 361 differentially expressed proteins, 157 upregulated proteins and 204 downregulated proteins in mature sperm relative to immature sperm. All of the identified proteins were categorized into seven types of subcellular localizations and three molecular functions and were found to be involved in nine biological processes. All of the differential proteins were involved in 235 different pathways. Moreover, we found that the tricarboxylic acid (TCA) pathway played an important role in the energy metabolism of sperm and that the EABB

SUBMITTER: Chen J 

PROVIDER: S-EPMC4786320 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature