Induction of secondary metabolism of Aspergillus terreus ATCC 20542 in the batch bioreactor cultures.

ABSTRACT: Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification of process conditions and growth media. Several strategies were tested, including the addition of rapeseed oil or inulin, changing the concentration of nitrogen source, reduction of chlorine supply, cultivation under saline conditions, and using various aeration schemes. Fifteen secondary metabolites were identified in the course of the study by using ultra-high performance liquid chromatography coupled with mass spectrometry, namely mevinolinic acid, 4a,5-dihydromevinolinic

SUBMITTER: Boruta T 

PROVIDER: S-EPMC4786612 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature