Dynamic modulation of phosphoprotein expression in ovarian cancer xenograft models.

ABSTRACT:

Background

The dynamic changes that occur in protein expression after treatment of a cancer in vivo are poorly described. In this study we measure the effect of chemotherapy over time on the expression of a panel of proteins in ovarian cancer xenograft models. The objective was to identify phosphoprotein and other protein changes indicative of pathway activation that might link with drug response.

Methods

Two xenograft models, platinum-responsive OV1002 and platinum-unresponsive HOX424, were used. Treatments were carboplatin and carboplatin-paclitaxel. Expression of 49 proteins over 14 days post treatment was measured by quantitative immunofluorescence and analysed by AQUA.

Results

Carboplatin treatment in the platinum-sensitive OV1002 model triggered up-regulation of

SUBMITTER: Koussounadis A 

PROVIDER: S-EPMC4787009 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature