The yeast genome undergoes significant topological reorganization in quiescence.

ABSTRACT: We have examined the three-dimensional organization of the yeast genome during quiescence by a chromosome capture technique as a means of understanding how genome organization changes during development. For exponentially growing cells we observe high levels of inter-centromeric interaction but otherwise a predominance of intrachromosomal interactions over interchromosomal interactions, consistent with aggregation of centromeres at the spindle pole body and compartmentalization of individual chromosomes within the nucleoplasm. Three major changes occur in the organization of the quiescent cell genome. First, intrachromosomal associations increase at longer distances in quiescence as compared to growing cells. This suggests that chromosomes undergo condensation in quiescence, which we confi

SUBMITTER: Rutledge MT 

PROVIDER: S-EPMC4787801 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature