Generation of Antigen Microarrays to Screen for Autoantibodies in Heart Failure and Heart Transplantation.

ABSTRACT: Autoantibodies directed against endogenous proteins including contractile proteins and endothelial antigens are frequently detected in patients with heart failure and after heart transplantation. There is evidence that these autoantibodies contribute to cardiac dysfunction and correlate with clinical outcomes. Currently, autoantibodies are detected in patient sera using individual ELISA assays (one for each antigen). Thus, screening for many individual autoantibodies is laborious and consumes a large amount of patient sample. To better capture the broad-scale antibody reactivities that occur in heart failure and post-transplant, we developed a custom antigen microarray technique that can simultaneously measure IgM and IgG reactivities against 64 unique antigens using just five microliters

SUBMITTER: Chruscinski A 

PROVIDER: S-EPMC4788148 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature