Pharmacological Modulation of Hemodynamics in Adult Zebrafish In Vivo.

ABSTRACT:

Introduction

Hemodynamic parameters in zebrafish receive increasing attention because of their important role in cardiovascular processes such as atherosclerosis, hematopoiesis, sprouting and intussusceptive angiogenesis. To study underlying mechanisms, the precise modulation of parameters like blood flow velocity or shear stress is centrally important. Questions related to blood flow have been addressed in the past in either embryonic or ex vivo-zebrafish models but little information is available for adult animals. Here we describe a pharmacological approach to modulate cardiac and hemodynamic parameters in adult zebrafish in vivo.

Materials and methods

Adult zebrafish were paralyzed and orally perfused with salt water. The drugs isoprenaline and sodium nitroprusside were

SUBMITTER: Bronnimann D 

PROVIDER: S-EPMC4788458 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature