Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon-sequencing libraries.

ABSTRACT: Porphyromonas gingivalis is a Gram-negative anaerobe and keystone periodontal pathogen. A mariner transposon insertion mutant library has recently been used to define 463 genes as putatively essential for the in vitro growth of P. gingivalis ATCC 33277 in planktonic culture (Library 1). We have independently generated a transposon insertion mutant library (Library 2) for the same P. gingivalis strain and herein compare genes that are putatively essential for in vitro growth in complex media, as defined by both libraries. In all, 281 genes (61%) identified by Library 1 were common to Library 2. Many of these common genes are involved in fundamentally important metabolic pathways, notably pyrimidine cycling as well as lipopolysaccharide, peptidoglycan, pantothenate and coenzyme A biosynthesi

SUBMITTER: Hutcherson JA 

PROVIDER: S-EPMC4788587 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature