Higher-Density Culture in Human Embryonic Stem Cells Results in DNA Damage and Genome Instability.

ABSTRACT: Human embryonic stem cells (hESC) show great promise for clinical and research applications, but their well-known proneness to genomic instability hampers the development to their full potential. Here, we demonstrate that medium acidification linked to culture density is the main cause of DNA damage and genomic alterations in hESC grown on feeder layers, and this even in the short time span of a single passage. In line with this, we show that increasing the frequency of the medium refreshments minimizes the levels of DNA damage and genetic instability. Also, we show that cells cultured on laminin-521 do not present this increase in DNA damage when grown at high density, although the (long-term) impact on their genomic stability remains to be elucidated. Our results explain the high levels

SUBMITTER: Jacobs K 

PROVIDER: S-EPMC4788786 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature