Protocol: transient expression system for functional genomics in the tropical tree Theobroma cacao L.

ABSTRACT:

Background

Theobroma cacao L., the source of cocoa, is a crop of significant economic value around the world. To facilitate the study of gene function in cacao we have developed a rapid Agrobacterium-mediated transient genetic transformation protocol. Here we present a detailed methodology for our transformation assay, as well as an assay for inoculation of cacao leaves with pathogens.

Results

Agrobacterium tumefaciens cultures are induced then vacuum-infiltrated into cacao leaves. Transformation success can be gauged 48 h after infiltration by observation of green fluorescent protein and by qRT-PCR. We clarify the characteristics of cacao leaf stages and demonstrate that our strategy efficiently transforms leaves of developmental stage C. The transformation protocol has hig

SUBMITTER: Fister AS 

PROVIDER: S-EPMC4788949 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature