Sub-optimal phenotypes of double-knockout mutants of Escherichia coli depend on the order of gene deletions.

ABSTRACT: Metabolic networks are characterized by multiple redundant reactions that do not have a clear biological function. The redundancies in the metabolic networks are implicated in adaptation to random mutations and survival under different environmental conditions. Reactions that are not active under wild-type growth conditions, but get transiently activated after a mutation event such as gene deletion are known as latent reactions. Characterization of multiple-gene knockout mutants can identify the physiological roles of latent reactions. In this study, we characterized double-gene deletion mutants of E. coli with the aim of investigating the sub-optimal physiology of the mutants and the possible roles of latent reactions. Specifically, we investigated the effects of the deletion of the glyox

SUBMITTER: Gawand P 

PROVIDER: S-EPMC4789104 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature