Selection of suitable reference genes for assessing gene expression in pearl millet under different abiotic stresses and their combinations.

ABSTRACT: Pearl millet [Pennisetum glaucum (L.) R. Br.] a widely used grain and forage crop, is grown in areas frequented with one or more abiotic stresses, has superior drought and heat tolerance and considered a model crop for stress tolerance studies. Selection of suitable reference genes for quantification of target stress-responsive gene expression through quantitative real-time (qRT)-PCR is important for elucidating the molecular mechanisms of improved stress tolerance. For precise normalization of gene expression data in pearl millet, ten candidate reference genes were examined in various developmental tissues as well as under different individual abiotic stresses and their combinations at 1 h (early) and 24 h (late) of stress using geNorm, NormFinder and RefFinder algorithms. Our results rev

SUBMITTER: Shivhare R 

PROVIDER: S-EPMC4789795 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature