Membrane Charge Directs the Outcome of F-BAR Domain Lipid Binding and Autoregulation.

ABSTRACT: F-BAR domain proteins regulate and sense membrane curvature by interacting with negatively charged phospholipids and assembling into higher-order scaffolds. However, regulatory mechanisms controlling these interactions are poorly understood. Here, we show that Drosophila Nervous Wreck (Nwk) is autoregulated by a C-terminal SH3 domain module that interacts directly with its F-BAR domain. Surprisingly, this autoregulation does not mediate a simple "on-off" switch for membrane remodeling. Instead, the isolated Nwk F-BAR domain efficiently assembles into higher-order structures and deforms membranes only within a limited range of negative membrane charge, and autoregulation elevates this range. Thus, autoregulation could either reduce membrane binding or promote higher-order assembly, dependin

SUBMITTER: Kelley CF 

PROVIDER: S-EPMC4790443 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature