A multistate tuberculosis pharmacometric model: a framework for studying anti-tubercular drug effects in vitro.

ABSTRACT:

Objectives

Mycobacterium tuberculosis can exist in different states in vitro, which can be denoted as fast multiplying, slow multiplying and non-multiplying. Characterizing the natural growth of M. tuberculosis could provide a framework for accurate characterization of drug effects on the different bacterial states.

Methods

The natural growth data of M. tuberculosis H37Rv used in this study consisted of viability defined as cfu versus time based on data from an in vitro hypoxia system. External validation of the natural growth model was conducted using data representing the rate of incorporation of radiolabelled methionine into proteins by the bacteria. Rifampicin time-kill curves from log-phase (0.25-16 mg/L) and stationary-phase (0.5-64 mg/L) cultures were used to assess t

SUBMITTER: Clewe O 

PROVIDER: S-EPMC4790616 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature