Prevalence and risk factors for carriage of ESBL-producing Enterobacteriaceae in Amsterdam.

ABSTRACT:

Objectives

The objectives of this study were to determine the prevalence of carriage of ESBL-producing Enterobacteriaceae (ESBL-E) in a representative sample of the general adult Dutch community, to identify risk factors and to gain understanding of the epidemiology of these resistant strains.

Methods

Adults enrolled in five general practices in Amsterdam were approached by postal mail and asked to fill in a questionnaire and to collect a faecal sample. Samples were analysed for the presence of ESBL-E. ESBL genes were characterized by PCR and sequencing. Strains were typed using MLST and amplified fragment length polymorphism (AFLP) and plasmids were identified by PCR-based replicon typing. Risk factors for carriage were investigated by multivariate analysis.

Results

SUBMITTER: Reuland EA 

PROVIDER: S-EPMC4790620 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature