Rare recombination events generate sequence diversity among balancer chromosomes in Drosophila melanogaster.

ABSTRACT: Multiply inverted balancer chromosomes that suppress exchange with their homologs are an essential part of the Drosophila melanogaster genetic toolkit. Despite their widespread use, the organization of balancer chromosomes has not been characterized at the molecular level, and the degree of sequence variation among copies of balancer chromosomes is unknown. To map inversion breakpoints and study potential diversity in descendants of a structurally identical balancer chromosome, we sequenced a panel of laboratory stocks containing the most widely used X chromosome balancer, First Multiple 7 (FM7). We mapped the locations of FM7 breakpoints to precise euchromatic coordinates and identified the flanking sequence of breakpoints in heterochromatic regions. Analysis of SNP variation revealed meg

SUBMITTER: Miller DE 

PROVIDER: S-EPMC4790991 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature