Expression of factor H binding protein in meningococcal strains can vary at least 15-fold and is genetically determined.

ABSTRACT: Factor H binding protein (fHbp) is a lipoprotein of Neisseria meningitidis important for the survival of the bacterium in human blood and a component of two recently licensed vaccines against serogroup B meningococcus (MenB). Based on 866 different amino acid sequences this protein is divided into three variants or two families. Quantification of the protein is done by immunoassays such as ELISA or FACS that are susceptible to the sequence variation and expression level of the protein. Here, selected reaction monitoring mass spectrometry was used for the absolute quantification of fHbp in a large panel of strains representative of the population diversity of MenB. The analysis revealed that the level of fHbp expression can vary at least 15-fold and that variant 1 strains express significan

SUBMITTER: Biagini M 

PROVIDER: S-EPMC4791009 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature