In Situ Quantification of Surface Chemistry in Porous Collagen Biomaterials.

ABSTRACT: Cells inside a 3D matrix (such as tissue extracellular matrix or biomaterials) sense their insoluble environment through specific binding interactions between their adhesion receptors and ligands present on the matrix surface. Despite the critical role of the insoluble matrix in cell regulation, there exist no widely-applicable methods for quantifying the chemical stimuli provided by a matrix to cells. Here, we describe a general-purpose technique for quantifying in situ the density of ligands for specific cell adhesion receptors of interest on the surface of a 3D matrix. This paper improves significantly the accuracy of the procedure introduced in a previous publication by detailed marker characterization, optimized staining, and improved data interpretation. The optimized methodology is

SUBMITTER: Tzeranis DS 

PROVIDER: S-EPMC4791220 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature